of Castile and Aragon, Duke of Burgundy (22 July 1478 – 25 September 1537) oo a) 1496
of Castile and Aragon (b. 6 November 1479)
1a) Eleanor of Austria (b. 15 November 1498) oo a) 1518
Manuel I of Portugal (31 May 1469 – 12 July 1523), b) 1524
Frederick II of the Palatinate (b. 9 December 1482)
1b) Eleanor of the Palatinate (15 May 1525 – 6 June 1529)
2b) Joanna of the Palatinate (b. 5 October 1526)
3b) Frederick of the Palatinate (b. 5 October 1526)
2a)
Charles V, Holy Roman Emperor, Duke of Burgundy (b. 24 February 1500) oo a) 1521 Sophia of Masovia (b. 3 January 1498)
1a) Eleanor of Austria (8 August 1523 – 24 May 1525)
2a) Joanna of Austria (b. 19 April 1527)
3a) Charles of Austria (b. 19 April 1527)
4a) Francis of Austria (b. 18 August 1529)
5a) Henry of Austria (stillborn 12 October 1530)
6a) Maximilian of Austria (b. 3 September 1531)
3a) Isabella of Austria (18 July 1501 – 5 August 1519) oo a) 1515
Christian III of Denmark (b. 1 July 1481)
1a) Hans of Denmark (b. 21 February 1518)
2a) Johanna of Denmark (b. 8 July 1519) o a) 1520 Henry Tudor, Prince of Wales (b. 10 November 1518)
3a) Annelise of Denmark (stillborn 8 July 1519)
4a)
Ferdinand of Austria, Prince of Asturias and Girona (b. 10 March 1503) oo a) 1521 Anna of Hungary (b. 23 July 1503)
1a) Isabella of Spain (b. 1 July 1522)
2a) Charles of Spain (stillborn 1 July 1522)
3a) Catherine of Spain (b. 5 July 1523)
4a) Ursula of Spain (b. 23 December 1525)
5a) Helena of Spain (b. 2 November 1526)
6a) Henry of Spain (stillborn 12 December 1527)
7a) Maria of Spain (b. 10 January 1529)
8a) Margaret of Spain (stillborn 26 October 1530)
9a) Alfonso of Spain (b. 18 August 1531)
10a) Ferdinand of Spain (b. 18 August 1531)
5a) Mary of Austria (b. 15 September 1505) oo a) 1515
Louis II of Hungary (b. 1 July 1506)
1a) Elizabeth of Hungary (stillborn 9 May 1521)
2a) Mary of Hungary (b. 8 March 1524)
6a) Catherine of Austria (b. 14 January 1507) oo a) 1523
John III of Portugal (b. 7 June 1502)
1a) Enrique of Portugal (b. 4 May 1526)
2a) Leonor of Portugal (stillborn 4 May 1526)
3a) Dinis of Portugal (stillborn 18 June 1527)
4a) Maria of Portugal (b. 6 February 1530)
5a) Felipe of Portugal (stillborn 20 January 1531)
2a) Margaret of Austria (b. 10 January 1480) oo a)
of Aragon, Prince of Asturias and Girona (30 June 1478 – 4 October 1497), b) 1501